We Dont Sell Foods, We Sell Health Research Paper - 2

We Dont Sell Foods, We Sell Health - Research Paper Example The target market for this ad is basically full-time housewife who wants taking good care of family and people who are interested in changing body condition by eating healthy foods. In this case, I choose to design this ad looks like a shopping receipt. My core idea is to tell customers Whole Foods Market sells health instead of foods and they buy more percentage to live well with the most competitive prices possible. According to Jenkins, an advert should capture the attention of the audience at a glance (Jefkins & Yadin, 2000). I used the font as the same one on the formal receipt in my ad because I want my audience to recognize it as a receipt immediately. The font color is black and the logo is green just like the Whole Foods current logo. The receipt also seems to be laying on the desk and a little curve in order to make it more stereoscopic and attractive. This feature enhances the clarity of the advert to the customer and brings out more attention and focus on the information provided. The final ad features â€Å"total health† in order to equip the knowledge to customers of the true nature of services available. It shows that complete health awareness is found here. The advert aims at increasing the awareness of customers of the significance of high-quality foods. This implies care on the part of the seller to the customers in promoting the quality and health in life. Vincent argues that an advertisement needs to be colorful and attractive depending on the media used. This captures the attention of the audience and sends a good message with clarity (Vincent, 1989). This receipt is suitable for magazine advertising since it lacks color and can be outstanding in the colorful magazine layout. The message is precise and the advert has been designed in a mode to fit in a small part of the magazine.  Ã‚  

Conflicting perspectives Essay Example for Free

Conflicting perspectives Essay Before initial reading: The comments to the right are my thoughts and questions on phrases I have placed in the essay and how appropriate they would be. Analyse the ways conflicting perspectives generate diverse and provocative insights. All texts composed convey an agenda which is based on the composer’s context. William Shakespeare’s Julius Caesar, Jason Reitman’s satirical film Thank You For Smoking (2005) and George Orwell’s Animal Farm (1945) all use conflicting perspectives to convey their agenda by generating diverse and provocative insights. Conflicting perspectives are generated through the use and potential abuse of power, manipulation and the contrast between public and private personas and thus the responder is positioned to accept the perspective the composer has deemed to be valid. The use and abuse of power, the use of manipulation to gain power and the contrast between public and private personas are explored through the portrayal of conflicting perspectives. It can be seen that the responder is positioned to †¦.. the point you’re trying to make is that the use of conflicting perspectives allows for some ambiguity in the portrayal of these themes but you might then say that ‘ultimately we are positioned to accept that†¦Ã¢â‚¬â„¢ and then you could specify exactly what. Shakespeare’s play Julius Caesar demonstrates conflicting perspectives on the nature of power. Shakespeare utilised this play as a commentary on the political situation of the time regarding the monarch of Elizabeth I. Elizabeth had gradually increased her power at the expense of the aristocracy and House of Commons, giving rise to political disputes. Likewise, Caesar has been granted dictatorial powers in the Roman Republic but he is not presented to have misused his power. Instead, Caesar’s hubris is more prevalent this which is seen to be the catalyst for the potential misuse of power. Brutus is aware of this and his idealism for a Roman Republic leads him to join the conspirators, shown in his soliloquy in Act Two Scene One. Brutus uses extended metaphors of a ladder and serpents in order to question Caesar’s ambition in conjunction with power. Brutus states  that ‘young ambition’s ladder [cause him to] look into the clouds, [leading him to] scorn the base degrees by which he did ascend’. Brutus also considers Caesar ‘a serpent’s egg, which would grow mischievous, and kill him in the shell’. The imagery of killing before birth reveals that Brutus is willing to remove Caesar before any tyrannical tendencies take place, suggesting that the assassination was unjust. Brutus would later attempt to justify the assassination that ‘because [Caesar] was ambitious, I slew him’. The conditional language and use of logos in demonstrating a clear cause and effect convinces the audience at Caesar’s funeral oration that the assassination was just, based on the declarative mood in their reaction that ‘Caesar was a tyrant’. However an alternative perspective is displayed through Antony. Even with Caesar’s dictatorial powers he has not seen any way in which he has abused his position, forming the basis of his defence of Caesar in his funeral oration. He uses conditional language by questioning ‘if Caesar was ambitious’ and he uses logos in presenting Caesar’s lack of ambition. Antony draws on examples such as ‘he thrice refused [the crown]’ and when coupled with the rhetorical question ‘did this in Caesar seem ambitious?’ he questions the view of the responders by providing a logical response to Brutus’ ill-based accusations. Antony is able to demonstrate Caesar’s lack of ambition and hence Caesar’s just use of power for the good of Rome. As a result Shakespeare presents two conflicting perspectives on power which generates diverse and provocative insights into his context. George Orwell’s Animal Farm also evokes similar ideas but its context as an allegory for the Stalinist rule of Soviet Russia must also be considered. George Orwell explores the notion of the corrupting nature of power in his allegorical novel (or political satire) ‘Animal Farm’. The allegory is a criticism of Soviet Communism tracks the rise of Napoleon, representing Stalin after the overthrow of Mr Jones, representing the last Tsar of Russia, Nicholas II. and how his power is seen to slowly corrupt prompting in its abuse. At the beginning of the allegory/fable the animals are seen to be harbouring discontent against their autocratic ruler of Mr Jones through their plotting to overthrow humans out of their farm. Major states with a declarative mood that they should ‘remove Man from the scene, and the root cause of hunger and overwork is abolished forever’. This connotes that Mr  Jones has abused his powers in allowing the poor conditions for his working animals. After the animals have revolted against Mr Jones the original commandments of Animalism are created, calling for the equal distribution of power as ‘all animals are equal’. However Napoleon’s use of intimidation through brute force upsets the equality and with the exile of Snowball Napoleon is left in a position of absolute power. Napoleon’s guard dogs are used as a motif to symbolise his power as even after his initial deployment of his dogs against Snowball ‘the dogs growled so threateningly that they accepted his explanation without any further questions’. Napoleon’s power is also seen to corrupt him and he is ironically presented as to be simply a replacement for Mr Jones. Through reverting the name of the farm back to ‘Manor Farm’ Orwell perceives that the situation the animals are in do not differ from the reality of life under Mr Jones. Napoleon’s ‘transformation’ into a man is seen to represent this as the animals ‘look from pig to man, from man to pig, and from pig to man again, but already it was impossible to determine which was which’. The repetition and inversion of the order of ‘pig’ and ‘man’ highlight the confusion the animals have over their present reality. This illustrates the corruption of power in Napoleon and with this Orwell is suggesting that life under Stalin was no different than from Russia’s previous autocratic ruler of Tsar Nicholas II. Consequently Orwell’s use of conflicting perspectives over the issue of power in an allegory is able to provide a commentary of Orwell’s perspective of the nature of Stalinist Russia. Good – you clearly analyse and show the changed perspective of Napoleon but revise and consider how to clearly identify the conflicting perspectives. Julius Caesar demonstrates how a conflict between public and private personas can result in conflicting perspectives of characters. Act One Scene One introduces the idea of conflicting perspectives within personas with the Senators Flavius and Murellius and their perception of the commoners. The Senators clearly see the commoners as inferior with their descriptions as of them as ‘vulgar’ and ‘the basest metal’. This juxtaposes the positive diction used by them to describe the commoners as ‘good countrymen’ when they are in the public sphere, suggesting that the patronising tone used had the sole purpose of gaining support from the commoners. However Antony is  the character that conceals the deepest division between public and private personas, demonstrating his Machiavellian nature. His public persona is presented toas supportive of the assassination by being supportive of the conspirators as he states that ‘it would become me better than to close in terms of friendship with thine enemies’. Give a bit more info of context. Who does he say this to? However his antithesis is indicated through his ‘hope that you shall give me reasons why and wherein Caesar was dangerous’. How? This is implied to be a signal of support from Antony towards the conspiratorshow? but it hid his inner motives of his desire offor conflict against the conspirators. His private persona is presented to be heavily opposed to the assassination and he calls for ‘havoc’ and ‘let slip the dogs of war’, with the violent imagery clearly stating his intention and perspective of the assassination. Mention that this is in a soliloquy before the oration and what we learn. His private persona is reflected in his funeral orations where he repeatedly implies his disagreement with the assassination but never explicitly stating his stance. Initially he states that he ‘comes to bury Caesar, not to praise him’, but this is juxtaposed with his questioning tone and use of rhetorical questions that serve the purpose of suggesting the lack of justice in the assassination. Eg? His private persona comes into full view by the conclusion of his oration where through pathos he calls the ‘stones of Rome to rise up and mutiny’. Link to the dogs of war comments.This perspective of encouraging mutiny is therefore in direct contrast with his interactions with the other conspirators. Explain where. As a result conflicting perspectives are shown also within the characters in order to generate diverse and provocative insights. Similarly, Jason Reitman’s satirical film Thank You For Smoking also projects similar ideas regarding public and private personas. The film is a satire onof the perception of cigarettes and tobacco but not to the extent in which it is condoned, due to the fact that no character in the film is seen smoking. Conflicts in private and personal personas are seen through the protagonist of Nick Naylor with his public support of cigarettes and questioning of its associated health issues, juxtaposed with his private persona where he openly admits to smoking-related health issues. This issue is displayed through Naylor’s criteria for winning an argument, that ‘I  proved that you’re wrong. And if you’re wrong, I’m right’extend this quote with film techniques used at this point. His public persona has a distinct lack of integrity as he provides false promises on the nature of his employer, the Academy of Tobacco Studies. This is shown at the beg inning of the film where on a talk show he promises ‘50 million dollars to encourage kids not to smoke’. This is done despite it being completely contradictory to the perspectives of his employer who asks him ’50 million dollars? Are you out of your mind?’ The anger of his boss on promising the sum of money is seen through the closeup of his face as well as his questioning tone. However Naylor’s private persona admits to the health issues associated with smoking. In a meeting with the MOD group (Merchants of Death, constituting people from America’s firearms, alcohol and tobacco industries) in the film they discuss deaths directly attributed to their industry in order to gauge America’s most hated industry. Naylor boasts that ‘my product puts away 475 000 a year’ and he gloats that the level of alcohol related deaths is miniscule in context ‘100 000 in a year? Wowee a tragedy. Excuse me if I don’t exactly see terrorists getting excited kidnapping anyone from the alcohol industry’. The unconcerned facial expression coupled with the mid-close up shots of Naylor displaying no emotion and the comparison of deaths to terrorism demonstrates the complete disregard of human life. You need to comment on the use of satire as a way to highlight the conflict between what is said on screen and the purpose of the film maker. This is exemplified by the fast transition of shots in the conversation indicating the competitiveness of the argument. This scene is a satire ?on the perceived lack of moral concern demonstrated by tobacco industry through its use of absurdist behaviour, mainly instigated by Naylor. Therefore Naylor’s contradiction between his public and private personas is also another medium used by Reitman in order to convey conflicting perspectives on his satire of the tobacco industry. Manipulation is one central concern in Julius Caesar which generates conflicting perspectives. Cassius is able to manipulate Brutus into firstly believing the idealism of his cause and thus joining the conspirators and enacting Caesar’s death. Brutus is presented to be an idealistic character as he ‘loves the name of honour more than I fear death’. The antithetical  language of ‘honour’ and ‘death’ demonstrates that he has cast himself as the honourable idealist, and when coupled with Brutus’ fear that Rome ‘chooses Caesar as their king’ Cassius is free to manipulate him into joining the conspirators. He uses hyperbole to exaggerate Caesar’s status and he juxtaposes images of him being both a ‘Colossus’ and a ‘sick girl’ in order to prove his weakness to Brutus. Cassius also appeals to his idealistic nature. His monologue contains the epistrophy epistrophe ‘one man’ that reveals Caesar’s true power, that only ‘one man is Rome’. This position is different to the democratic Roman Republic that Brutus represents and admires and he is slowly manipulated to join the conspirators. Manipulation is also seen to demonstrate the power of language, seen through the differing funeral orations of Brutus and Antony. Initially the commoners are irate onabout Caesar’s assassination and the declarative mood presented through ‘we will be satisfied’ highlights their anger but it also serves as a point of juxtaposition. Brutus’ use of logos convinces the crowd that he ‘loved Rome more’ than Caesar through posing rhetorical statements that question the loyalty of the commoners and Caesar’s supposedly true nature. Immediately following his oration the commoners immediately affirm that ‘Caesar was a tyrant’, with this declarative statement juxtaposing heavily with the sentiment displayed in the first line of the scene. However the crowd are manipulated again by Antony to subscribe to his point of view. He adopts a questioning tone throughout his oration in inquiring onabout the validity of Brutus’ statements in order to not directly oppose Brutus but let his audience question their own perspectives. He repeats the phrase ‘Brutus says he was ambitious, and Brutus is an honourable man’ but the repetition of the phrase combined with the logos in his argument in proving Caesar’s lack of ambition therefore hint at the invalidity of Brutus’ argument. Further on in his oration Antony also uses metaphor to describe Brutus, that he was ‘Caesar’s angel’. This image is contrasted with the assassination, the ‘most unkindest cut of all’ and this contributes to the sentiment of betrayal with Brutus that assists in manipulating the audience. By the conclusion of the speech the commoners are united with one common purpose, shown in the stage direction in that all the commoners state ‘We’ll mutiny [against Brutus]. Anto ny’s rapid manipulation of the crowd demonstrates the power of language  and how it can be used to shape perspective. The manipulation of the animals in Animal Farm also conveys similar sentiment. Orwell utilises a cyclical structure of the allegory in order to demonstrate the power of manipulation instigated mainly by the antagonist of Napoleon and his spokesperson of Squealer. Orwell’s use of the text as an allegory of Soviet Russia creates a chilling context for the reader due to the fact that manipulation was repeated in Stalin’s rule of Russia, leading to the subjugation of the citizens of the Soviet Union. The pigs of Napoleon and Snowball quickly establish themselves as the superior class in the new ‘classless’ society of Animalism. Their superior intellect immediately allows them to create a simplified maxim for Animalism, which was to be ‘four legs good, two legs bad’. The sheep, representing the uneducated class, immediately begin to circulate this simplified form of Animalism and their attitude is reflected in the sheep ‘bleating this onfor ho urs on end’. The onomatopoeia exemplifies the repetitive nature and submissiveness in accepting this maxim and thus of the commands set by Napoleon throughout the allegory. Motif as well? The commandments of Animalism is used as a vehicle to demonstrate the manipulation of the animals. The commandments are changed repeatedly throughout the allegory to manipulate the common animals and their ‘poor memory’ as well as their naivety contributes to their ultimate acceptance of the new norm. The first notion of this manipulation is done when the pigs move and reside in the farmhouse. The new commandment reads ‘no animal shall sleep in a bed with sheets’, with the ‘sheets’ clause added. The character of Clover demonstrates the naivety of the animals in general as she ‘had not remembered if it mentioned sheets, but as it was there on the wall, it must have done so’. The high modality of ‘it must have done so’ contrasts her failure to rememb er the commandment. Squealer’s immediate entry in justifying the change in the commandment is another example of manipulation as he uses his intellectual superiority and logos in order to influence his listeners. He states that a bed ‘is simply a place to sleep in’ and not a physical construct and his argument juxtaposes the affirmation at the establishment of Animalism that the commandments were ‘unalterable laws’. The death of the character of Boxer also reveals the extent of Napoleon’s manipulation. Boxer is a follower of Napoleon throughthe adoption of the motto ‘Napoleon is always right’ which, when combined with his hardworking nature, should warrant great care and respect from the pigs. However when Boxer falls ill he is taken to the ‘horse slaughterer’ to be killed in exchange for money. This reality, witnessed by the animals, contrasts the explanation of Squealer in that ‘the van had previously been the property of the knacker, and had been bought by the veterinary surgeon’. By the conclusion of the allegory Napoleon’s power has become increased to such an extent that even the commandments of Animalism are shown to reflect the changes in that ‘all animals are equal, but some animals are more equal than others’. The paradoxical nature of this statement juxtaposes the original idealistic thinking based behind the creation of Animalism and demonstrates the extent of the manipulation of the animals. The animals are not aware that they are living in conditions very similar to life under Jones which forms the basis of Orwell’s cyclical structure of the allegory. The bending of the truth by the pigs as well as the willingness of the animals to accept this reality highlights the vast extent and impact of manipulation. Thus manipulation is another medium in which conflicting perspectives can be revealed. Composers of texts use conflicting perspectives in order to generate diverse and provocative insights into their context. These perspectives are shown to favour one side and they can provide an insight into the opinion of the composer on issues affecting their context. This is seen in Julius Caesar, Thank You For Smoking and Animal Farm where the composers, through their portrayal of power, public and private personas and manipulation that form the basis of conflicting perspectives. This is excellent overall. However, there are things you can do to improve. The analysis of AF is almost longer than Caesar. You must mention the end of Caesar in your analysis. There doesn’t seem to be at least one quote from Acts 4 or 5. Find a couple and use them. In the first body paragraph on power, use quotes for Antony from elsewhere in the play, not just the oration. Eg comment on the deification of Caesar at the start and how stage directions are used by Shakespeare to contrast the views of the commoners/Antony/ and Cassius/Brutus and others. Give an example of where we  see Caesar’s hubris ‘northern star’†¦ and contrast that with his humility elsewhere. Shakespeare is always offering contrasting views and creating ambiguity. However, comment also on how we are finally positioned at the end of the play. If discussing Brutus’s hubris, mention the tragic structure. Contrast the way this character is presented at the beginning, middle and then the end when we see the consequences of both his idealism and hubris when he fights with Cassius and dies and then the final comments by Antony. When writing about Antony, consider the way he is presented as loyal, then angry and vengeful about the murder of Caesar, then pitiful, then scheming and manipulative. Take quotes from a range of places throughout the whole play to give the reader a better understanding of the narrative arc of this character. The final paragraph on AF could be edited. It is very long and veers away from the idea of conflicting perspectives in the middle and becomes an analysis of the novel. Throughout the discussion, you could make links between it and Caesar to remind the reader of your point. Email if you have questions.

Side Effects of Tumor Size Reducing Drugs | Experiment

Side Effects of Tumor Size Reducing Drugs | Experiment Manish Kumar Tiwari 1. Introduction: Objective: Pfizer have developed a new drug that appears to reduce the size of specific tumors but are concerned about what effect the drug might have on normal tissue. Outline how you would use DNA technology to address this issue. Cancer disease has large complexities in terms of genome variations at genetic level and epigenetic level. Immortalization and tumor genesis are the two fundamental characteristics of cancerous cells. This disease is caused by mutations in genes such as oncogenes, DNA repairing genes and tumor suppressor genes. Recent researches suggested that more than one mutations are needed for the cancers. One of the major drawbacks of the medicines or drugs that are used to treat cancer is its side effects on normal cells. The cells which are mostly affected by drugs are rapidly dividing cells such as blood cells, hair follicles cells, cells found in tract of reproductive organ and digestive system, and cells from immune system. Side effects on normal cells due to chemotherapy has become major challenges for researchers. Transcriptome or protein expression profiling for cancerous cells treated with specific drugs may provide useful information about possible side effects on normal cells. When a ny drugs or medicines are given for the treatment of any specific tumor disease, it binds with specific receptors (cell surface receptors, Cytoplasmic receptors or nuclear receptors) and leads to transcription and translation process and generate specific proteins that can be able to stop the cell cycle or initiation of apoptosis. But generally these drugs may also responsible to translation of unwanted proteins that can cause side effects on normal tissues. 2. Approach: Mode of action of many drugs that reduces the size of tumor are related to growth cycle (like mutagen, MAP kinase pathway) or DNA modifications (transcription, translation etc.). This method is more suitable for in vivo testing in rats or mammalian cancerous cell lines which has been described here. The added tumor size reducing drug must bind with specific receptors on the tumor cells. So first step is the identification of pathway via which it acts. In the downstream signaling of the pathway, some transcription factors will be activated and will bind to target promoter and reduces the size of tumor. So then transcription factors need to be quantified by qPCR as well as the sequence of their promoter through DNA Foot Printing. Now two plasmids need to be constructed (minimum two plasmids, if there are more transcription factors and promoters, more plasmids with different fluorescent proteins are needed) containing above identified promoter coupled with red fluorescent protein (RFP) and containing a tumor inducible promoter coupled with green fluorescent protein (GFP). Now for in vivo testing, mutant mouse are created and transfected with above two plasmids. During the growth, the known tumor inducing compounds/radiation is given to the mouse to induce the tumor. As the drug is added, it will cause induction of RFP through the body but level may be higher in tumor cells but GFP should be induced only in the tumor cells. If GFP is induced in other normal cells it means that this drug may cause side effect on that cells. A fluorescent mapping of mouse will reveal the efficacy and side effects of drug based on RFP and GFP intensity.  Ã‚   Figure 1: Schematic presentation of approach. The image of mice is taken from internet which has been used to explain the method. 3. Method: This method is suitable for in vivo testing in mice or mammalian cells culture. The main steps include quantification and identification of transcription factors and promoter sequences respectively, construction of suitable plasmids coupled with red and green fluorescent proteins, transfection of plasmids in mice body, tumor induction in mice body followed by drug injection and last fluorescent mapping using fluorescent detector. The instruments and techniques which will be used in this methods are qRT-PCR, DNase Foot Printing assay, Suitable plasmids vector, microinjections, Chemicals, fluorescent proteins (red and green), capillary electrophoresis, tumor inducing cells or chemicals or radiation and fluorescent detector. Validation of this method is important so validation could be possible by using this method for any known drug which side effects on normal cells has been identified completely. 3.1. Quantification of Transcription Factor: The exact quantification of transcription factor is the most important part of this method. Micro array or PCR is the good technique for quantification of transcription factors but in this method qPCR/QRT-PCR will be appropriate technique. First step is isolation of cancer cells from mammalian cancerous cell lines. Then inject target anti-cancer drug and incubate for some time because these drugs takes some time to start their function. After proper incubation, total or poly A RNA extraction is the next step. The solution which is used in extraction process should be RNase free otherwise it can degrade our RNA so that exact quantification could not be possible. Sample should be treated with DNase to remove genomic DNA contamination. Flow Chart 1: Steps involved in quantification of Transcription factors Electrophoresis and qPCR methods could be used for determination of purity and accurate concentration because these factors are very important for proper gene expression profiling. Then C DNA synthesis and validation of C DNA quality and quantity could be done by using qRT-PCR. For performing qRT-PCR assay there are two important steps such as selection of appropriate reference genes and designing of PCR primer labeled with fluorescent dye must be needed. For data analysis fluorescent detector can be used to detect transcription factors and their associated genes. Now once genes have been identified by using above method so the identification of their promoter sequence DNA Foot Printing assay will be performed. 3.2. Identification of Promoter Sequence: DNase Foot printing assay method can be used to identify target promoter sequence. Steps involved in this method is amplification of target DNA through PCR using fluorescent labeled primer at 5’ end. Then cleavage of the amplified DNA by using DNase enzyme followed by the capillary electrophoresis. The cleavage pattern will vary due to the presence of transcription factor, because the binding sites are protected by the protein from the cleavage. By using this method we can identify the promoter sequences. By using capillary electrophoresis we can identify the amount and size of DNA fragments and about the bases which are not cleaved by the DNase enzyme. Figure 2: Identification of promoter sequences through DNA Foot Printing assay. The graph between amount and size of DNA fragments in this figure is showing the bases which are protected by the transcription factor against DNase enzyme. 3.3. Construction of Suitable Plasmids: Construction of suitable expression vectors for mammalian cells, that can carry the desired promoter sequence coupled with fluorescent protein must be needed. The most important characteristics of vectors is presence of all elements that is suitable for expression in host cells. The important elements are promoter, stop and start codon, binding sites for ribosome, ORI region and appropriate selection markers. Some examples of vectors like adenoviral, PSV and pCMV are generally used for expression in mammalian cells. In this method, our expression vectors should contain promoter sequence labeled with red and green fluorescent protein and other important elements. Minimum two type of plasmid vectors need to be constructed. One plasmid should have promoter coupled with RFP which has not induced by the tumor inducible transcription factors. Other plasmid should have tumor inducible promoter coupled with green fluorescent protein. Our main idea is to inject these vectors into the mutated mice body so that we also need to remove the other elements of vectors that can cause any unwanted diseases in mutated mice. The vectors like pED and Pz can be used for the expression in mammalian cells. Figure 3: Construction of plasmids containing promoter coupled with Red and Green fluorescent protein. The very first step for the construction of the recombinant plasmid is the cleavage of both plasmid and target DNA with promoter sequence coupled with fluorescent protein using suitable restriction enzymes. The restriction enzymes creates sticky or blunt ends (depends on type of restriction enzyme used) in both plasmid and target DNA. Next step is the hybridization of both DNA and plasmid using DNA ligase enzyme. Selection of cells having plasmid with desired sequence is very important so further we need to selection of appropriate vector by using selection markers like antibiotic resistance genes. 3.4. Transfection: The transfer of desired plasmid inside the mice body could be possible through many ways such as microinjection, electroporation, shotgun method, through chemicals and viral infections. Transfection through viral infection has some limitations like limited carrying capacity of desired gene and unwanted inflammatory mutations. However, transfection through viral infection have some advantages like easy to handle, easy preparation and easily monitoring during the process. So, in this method transfection of plasmid in mice should be done directly through microinjection into the mice body. One another way for transfection of recombinant plasmids in mice is through recombinant Baculovirus. Baculovirus infects insect cells. Purified budded virus can be isolate from the infected insect cells with recombinant Baculovirus. This purified budded virus can be introduced inside the mice body. For the study of side effects on normal cells in whole body of mice it is very important that this recomb inant plasmids will reach every parts of body along with tumor affected parts. 3.5. Induction of Tumor in Mice: Mammalian cancerous cell lines or cell DNA extracted from virally infected cells can be able to induce cancer in mice. Once theses tumorgenic cells is injected inside the mice body it develop specific tumor. After developing cancer in mice body, anti-cancer drug is administered through injection to show the efficacy and side effects on cancerous and non-tumor cells. When drugs binds with specific target receptors, it will induce both promoters but with varying intensity. The promoter coupled with RFP will show intensities in both normal and tumor cells but may be higher in tumor cells. But GFP should be induced only in tumor cells if it is inducing in other normal cells with high intensity then it may cause side effects on those normal cells. 3.6. Fluorescent Mapping: Analysis of fluorescent mapping of these promoters in different locations of the mice body can provide useful information about possible side effects against designed anti-cancer drugs. For example if GFP will be induced in other cells like hair cells, heart cells, bone marrow cells than we can predict the side effects on these cells because the drug should not induce translational process in normal cells. If this drug induces promoters only in tumor cells then the chances of side effects may be less. We can study possible side effects against various drugs by using this method. Figure 4: This picture has been modified for illustrating the possible results that can be produced by this method. Region B and C in this figure are representing the cancerous cells where GFP has been expressed. Region A is representing the normal cells where GFP has been also expressed so this drug may cause side effects on this cells. 3.7. Validation of the Method: This approach has not been validated because this is the hypothesis only. For the testing of this method whether it is working efficiently or not need to be validated. An efficient approach has been described here. For the validation of this method we need to perform this method on known anti-cancer drugs for specific type of cancers. This method can be apply for known drugs which side effects on normal cells have been identified completely. If fluorescent mapping provide exact location in the body where GFP has been induced and if these locations are related with those areas where this specific drug causes side effects then this method will be validated. But proper validation need to be tested for various anti-tumor drugs which side effects has been completely known. 4. Discussion: There are so many side effects associated with anti-cancer drugs because these drugs mainly affects rapidly dividing cells and immune system. The drugs or medicines that are currently used have always some common side effects like typhlitis, diarrhea and hair loss but sometimes these drugs cause serious side effects like liver damage and cardiac arrest because these drugs are unable to differentiate rapidly growing normal and cancerous cells. So that development of proper efficient method for testing possible side effects for any anti-cancer drugs should be developed. In this section a good approach has been described for the identification of possible side effects on normal cells. The idea is based on the role of transcription factors induced by the drug- receptors interactions. As instance certain anti-tumor drugs causes anemia when used for the treatment of specific tumor. Generally the gene called HBB is responsible for anemia because this gene encode beta globins protein. It mea ns that these drugs also induces transcription factor that is responsible for activation of HBB gene. The fluorescent mapping of unknown anti-cancer drug against specific cancer can provides useful information about possible side effects. The figure 4 which has been modified to illustrate the possible results that can be achieved through this method. If the drug is not inducing GFP in normal cells except cancerous cells it means drug will not cause any side effects on normal cells but vice versa if GFP is expressing in other cells along with tumor cells so we can predict possible side effects on those cells because this method is also useful to find out what type of protein or transcription factors are expressed. By using bioinformatics data bases like PDB, Gene bank etc, functions of expressed proteins or transcription factors can be easily predict. The method which has been described above has not validate yet because this method is only a hypothesis that need further advancement and validation. 5. References: Lohmann, S., Herold, A., Bergauer, T., Belousov, A., Betzl, G., Demario, M., Dietrich, M., Luistro, L., Poignà ©e-Heger, M., Schostack, K., Simcox, M., Walch, H., Yin, X., Zhong, H. and Weisser, M. (2013). Gene expression analysis in biomarker research and early drug development using function tested reverse transcription quantitative real-time PCR assays. Methods, 59(1), pp.10-19. Swartzman, E., Shannon, M., Lieu, P., Chen, S., Mooney, C., Wei, E., Kuykendall, J., Tan, R., Settineri, T., Egry, L. and Ruff, D. (2010). Expanding applications of protein analysis using proximity ligation and qPCR. Methods, 50(4), pp.S23-S26. Genetics Home Reference, (2014). HBB gene. [online] Available at: http://ghr.nlm.nih.gov/gene/HBB [Accessed 23 Nov. 2014]. Dubensky, T., Campbell, B. and Villarreal, L. (1984). Direct transfection of viral and plasmid DNA into the liver or spleen of mice. Proceedings of the National Academy of Sciences, 81(23), pp.7529-7533. Caldana, C., Scheible, W., Mueller-Roeber, B. and Ruzicic, S. (2007). A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors. Plant Methods, 3(1), p.7. Kim, T. and Eberwine, J. (2010). Mammalian cell transfection: the present and the future. Analytical and Bioanalytical Chemistry, 397(8), pp.3173-3178.

The Feudal Society of Matewan :: Stone Mountain Coal Company Economics Essays

The Feudal Society of Matewan The Stone Mountain Coal Company wielded monopoly control over the town of Matewan through a feudal system of economic, cultural, political, and environmental processes. Every person in the town of Matewan came under the power of the company in one way or another. The employees of Stone Mountain were under a bondage contract with the company. Once they came to the company it was impossible to leave and at the same time maintain a basic standard of living. They could not leave also because once they signed on with the company they owed the company a large sum of money for everything ranging from the ticket for the train that brought them to Matewan to the equipment they needed to work there. In this way it was as if they were paying the company to let them work in the mines rather the other way around. They did not have the rights basic to capitalism such as the right to join a union, the right to sell their labor in a labor market to the bidder of their choice, and the rig ht to spend their wage freely. The Stone Mountain Coal Company controlled the processes involving the production, circulation, and distribution of products and services in the town of Matewan. Most of the people in the town were employees of the company and others were in debt to the company through mortgages and loans. Everyone depended on the company for daily necessities such as food and clothing because the company owned the stores in Matewan and because all employees of Matewan were paid in company script which only allowed them to buy in the company stores. Furthermore, their contract said that to buy from any other store would mean immediate discharge from employment. The Stone Mountain Coal company was the only major employer in town. To become self-employed, citizens of Matewan would have needed initial endowments of resources, products and land that were only available at the company's prerogative. The Stone Mountain Coal company kept economic control through political processes which served to punish behavior unfavorable to the company. The company sent in agents from Baldwin-Felts to act as the feudal managers or Knights who intimidated the people and infiltrated the new union.

Its Time to End the Drug War Essay -- Argumentative Persuasive Essays

It's Time to End the Drug War Uhh, uhhh B.I.G., P-O, P-P-A No info, for the, DEA Federal agents mad cause I'm flagrant Tap my cell, and the phone in the basement -Notorious B.I.G. lyrics from â€Å"Mo’ Money, Mo’ Problems† In Christopher Wallace’s (a.k.a. Notorious BIG) â€Å"Mo’ Money, Mo’ Problems†, the late rapper from Brooklyn mentions his run in with the police earlier in his life. Christopher Wallace came to be known as arguably the greatest rapper the world has ever heard, but before the days as a famous entertainer Christopher Wallace an average crack dealer in New York. Many youths in the New York area wish to follow his path, and sell drugs in their respective neighborhoods in order to be able to â€Å"roll in style.† Some individuals would try to say that the current system for dealing with drugs works in this country, look what it did for Christopher Wallace how he straightened up his life after jail. That could be the furthest from the truth, if one were to look at the rest of Christopher Wallace’s songs, one could deduct the violence, and anger that has built up in him. Christopher Wallace’s life would come to an abrupt and violent end , when he would be shot down in a drive-by in Los Angeles at the age of 24. The tragic end of such a talented and troublesome life, brings me to ask the question whether the US’s war on drugs is actually accomplishing anything, when drugs are still easily available in metropolitan cities across the country, especially New York. The modern Drug War’s roots can be dated back to US anti-imperialist sentiments against the British since the 19th century. More recent incarnations of these sentiments are figures such as Richard Nixon, Harry Anslinger, and George Bush. Drugs such as ... ...t.all; â€Å"Estimating Heroin Availability†. Cambridge, Mass: Abt Associates Inc.; 1999 9. http://www.csdp.org/edcs/theneed.htm 10. http://www.whitehousedrugpolicy.gov/publications/policy/ndcs00/chap2_4.html 11. http://www.progressive.org/com1099.htm 12. Wisotsky, Steven; Breaking the Impasse in the War on Drugs. New York:Greenwood Press; 1986 13. Martin, Susan Taylor; â€Å"Heroin for addicts works as a Swiss fix†. St. Petersburg: St. Petersburg, FLA. 2001 14. Reinarman, Craig and Cohen, Peter. â€Å"Is US dutch policy the devil?†. Univeristy of Amsterdam :Amsterdam; 1999. 15. Donrey, Samantha Young. â€Å"US:DEA Profile Being Raised by New Head. Wahington: Donrey Media Group; 2001 16. Hunt, Gibson Hunt and Chambers Carl D. â€Å"The Heroin Epidemics†. New York, Spectrum Publications; 1976 17. http://www.dea.gov/concern/heroin.htm 18. http://www.drugwarfacts.org/thenethe.htm

Essay --

Budgetary shortfalls have become a part of everyday news and a grim reality for both public and private sectors. Negotiating a win-win scenario for all vested parties sometimes can seem like an insurmountable and daunting task. In order to run a successful municipality, all vested parties must collaborate and be solution based. Transparency plays a huge role in establishing trust, credibility and facilitating an agreeable end result. Every financial organization must create a budget. In simple terms, the budget is a plan that outlines how resources will be utilized during a specific period of time. A balanced budget generally translates into an organization demonstrating financial health and or responsibility, (Kennon, 2014). When the amount of goods or services exceeds the amount of revenue being generated, budgets then need to be revised so that they are in line. This generally means that concessions need to be made by governmental officials, union groups, employe es, constituents or all of the aforementioned stakeholders in the community. Due to a drop in revenue, a slight increase in contracted services and an increase in post employment expenditures for the current fiscal year, the mayor of my jurisdiction has asked all department heads to analyze, revise and submit a budget reflecting a 2% decrease in expenditures for the following year. This will allow for the government to remain solvent without raising the tax rate. As the department head of the public safety division, I oversee the budgets for the police and fire departments. This includes 44 full time police officer, 16 full time fire fighters and 10 part time fire fighters. Total yearly expense for the public safety division is $9,460,000 or 72% o... ... this would reduce the number of calls and offset the expense by 25% or $18,750. Each year the police and fire departments are involved in community outreach efforts. Costs in excess of $31,500 for events like the national fire safety week, national night out, crime stoppers, DARE, personal safety seminars and workshops, etcetera are typically absorbed by the public safety department and provided to the community free of charge. Being strategic as to what educational programs are offered and limiting the events to quarterly rather than monthly would save the jurisdiction approximately $7,000 annually without completely eliminating the programs that the community has come to expect. All told with the strategic approach to budget allocation and the implementation of targeted increases for those who violate the law, the proposed savings is $190,250. Essay -- Budgetary shortfalls have become a part of everyday news and a grim reality for both public and private sectors. Negotiating a win-win scenario for all vested parties sometimes can seem like an insurmountable and daunting task. In order to run a successful municipality, all vested parties must collaborate and be solution based. Transparency plays a huge role in establishing trust, credibility and facilitating an agreeable end result. Every financial organization must create a budget. In simple terms, the budget is a plan that outlines how resources will be utilized during a specific period of time. A balanced budget generally translates into an organization demonstrating financial health and or responsibility, (Kennon, 2014). When the amount of goods or services exceeds the amount of revenue being generated, budgets then need to be revised so that they are in line. This generally means that concessions need to be made by governmental officials, union groups, employe es, constituents or all of the aforementioned stakeholders in the community. Due to a drop in revenue, a slight increase in contracted services and an increase in post employment expenditures for the current fiscal year, the mayor of my jurisdiction has asked all department heads to analyze, revise and submit a budget reflecting a 2% decrease in expenditures for the following year. This will allow for the government to remain solvent without raising the tax rate. As the department head of the public safety division, I oversee the budgets for the police and fire departments. This includes 44 full time police officer, 16 full time fire fighters and 10 part time fire fighters. Total yearly expense for the public safety division is $9,460,000 or 72% o... ... this would reduce the number of calls and offset the expense by 25% or $18,750. Each year the police and fire departments are involved in community outreach efforts. Costs in excess of $31,500 for events like the national fire safety week, national night out, crime stoppers, DARE, personal safety seminars and workshops, etcetera are typically absorbed by the public safety department and provided to the community free of charge. Being strategic as to what educational programs are offered and limiting the events to quarterly rather than monthly would save the jurisdiction approximately $7,000 annually without completely eliminating the programs that the community has come to expect. All told with the strategic approach to budget allocation and the implementation of targeted increases for those who violate the law, the proposed savings is $190,250.

Student: Human Resources

Hitek 1 Title: JOB DESCRIPTIONS AT HITEK & DOWNSIZING: ANATHEMA TO CORPORATE LOYALTY Institution: Grantham University Instructor: Barbara Davis Student: Franessa Wilson Date: March 26, 2013 Hitek 2 Case Study: Job Descriptions at Hitek Based on what you know about high-tech companies, what are some likely strategic objectives for HITEK? Given these what should be the objectives of HITEK’s HR department? Do you think these are the objectives that guide the behaviors of Rains? Explain. A strategic objective is one that an organization must achieve to make its strategy succeed.Strategic objectives for Hitek are continued industry issues and supply chain challenges of prices, short product lifestyles, mass customization, globalization and strategic market planning. Strategic objectives provide direction for an organization, and strategic objectives provide a way to measure the organization’s progress toward realizing the ideas listed in the mission statement. For instance, if an organization has the idea that it will provide perfect customer service, then a strategic objective for the organization would be to score at the top level on customer service satisfaction.Strategic human resource objectives are goals aligned with the organizations goals. It’s the managerial process requiring human resource policies and practices to be linked with the strategic objectives of the organization. The human resources objectives at Hitek, supports organizational goals; such as profitabilities, business regulations, ethics and principles. Yes, I believe these are the objectives that guide the behaviors of Rains. Isabel Rains is the vice president of human resources and she rules the department with iron fist.Iron fist according to Wikipedia means with strict authority. Hitek 3 Jobs change frequently at HITEK. What approach to job analysis makes the most sense in such a fast changing environment? Customized? Standardized? Task-focused? Competency modeling? Eval uate the strengths and weaknesses of the alternative approaches and make recommendations to Hill about how to proceed. A job analysis sits at the heart of all human resource practices making it a critical component of management activity in every organization.Emphasis should be placed on a strategic approach to job analysis, present a strategic job analysis framework and discuss implications for the organization. A job analysis is necessary for the successful functioning of an organization. Job analysis is focused on the collection of work related information for the job as it currently exists or existed in the past. The need for change often is unpredictable, thus tends to be reactive. The successful management of change is crucial to an organization in order to survive, and succeed in the present highly competitive, and continuously evolving business environment.The environment of an organization consists of the conditions circumstances and influences that affect the organizationà ¢â‚¬â„¢s ability to achieve its objectives. The external and internal environment of an organization are both composed of five elements; which are physical, technological, social, political, and economical. The external environment influences how human resource functions will be performed. The internal environment influences both of human resource policies and procedures, as well as the individuals who make up the workforce of the organization or business.External environment has a significant impact on HR policies and practices. It helps to determine the values, attitudes, and behavior that employees bring to the job. Internal environment is also Hitek 4 known as organizational climate. The elements of the internal environment technological, physical, social, political, and economical effect and are affected by the policies, procedures, and employment conditions that managers oversee. The human resource department plays a more active role in influencing change in an organization or business.Organizational effectiveness is critical to success in any economy. Change management is defined as the process of continually renewing an organizations direction, structure, and capabilities to serve the ever changing needs of the internal and external customers or consumers. According to Burnes 2005, change is an ever present feature of organizational life, both at an operational and a strategic level. In order to achieve increased and sustainable business results, organizations need to execute strategy and engage employees.To create organizational effectiveness, business leaders need to focus on aligning and engaging their people, the people management systems, and the structure and capabilities (including organizational culture) to the strategy it results in higher financial performance, higher customer satisfaction, and higher employee retention. An organization that can sustain such alignment will achieve increased business results. Effective implementation of strate gy is a key driver of financial performance. Organizations that fail to fully engage their workforce in the business strategy will fail to produce reliable, sustainable business results.The link between employee engagement factors and successful strategy execution is vital (Burnes, 2005). Hill could use a SWOT analysis for the strengths and weaknesses of an alternative approach. SWOT stands for strengths, weaknesses, opportunities, and threats. A SWOT analysis can offer helpful perspectives at any stage of an effort. Explore possibilities for new efforts or solutions to problems. Make decisions about the best path for the firm’s initiative. Identifying the opportunities for success in context of threats to success can clarify directions and Hitek 5 choices.Determine where change is possible. If the organization is at a juncture or turning point, an inventory of their strengths and weaknesses can reveal priorities as well as possibilities. Adjust and refine plans mid-course. A new opportunity might open wider avenues, while a new threat could close a path that once existed. The purpose of performing a SWOT is to reveal positive forces that work together and potential problems that need to be addressed or at least recognized. Before conducting a SWOT analysis, Hill should decide what format or layout he will use to communicate the issues.Strengths are resources that an organization possesses and the capabilities that an organization has developed. Weaknesses are resources and capabilities that are lacking or deficient in an organization; thus preventing an organization or business from developing a sustainable competitive advantage. Is the new job description (case exhibit 4) better than the old one (case exhibit 3)? Why or why not? Consider the perspective of employees as well as the perspective of the person supervising this job, Does your answer change depending on the way the job description is being used?The new job description is better than the old job description because it is more detailed. The skills needed are listed, how much education the applicant should have, and how much relevant experience is required. The supervision received is listed, therefore the applicant knows that he or she will be working under close supervision of a technical superior or manager, but no supervisory duties or experience is required. No, my answer does not change. Hitek 6 Case Study: Downsizing- Anathema to Corporate Loyalty Why is Daniels sensitive to DSI’s recruitment efforts?At Defense Systems Incorporated, Daniels feels that DSI’s layoffs or terminations are poor alternatives to dealing with a turbulent environment. The major problem as Daniels sees it is to preserve as many jobs as possible until business picks up. To terminate the new hires would irreparably harm DSI’s future recruitment efforts. Although terminations would improve the balance sheet in the short run, Daniels worried about the impact of such a move on corporate loyalty, a fragile and rare commodity at other major firms that have had to cut their white collar workforce ( Jackson et al; 2009, p. 226).A great possibility for downsizing to be an unsuccessful firm's strategy and therefore to decrease the corporate reputation, it is important before making the decision about downsizing to reconsider the necessity of such a decision. Downsizing has become a critical issue around the world. Downsizing and mass lay-offs are happening not only on US companies but also organizations in the entire industrial world. The number of organizations and jobs affected by downsizing has been staggering. In theory, downsizing is presumed to have positive outcome for an organization. In many situations, downsizing did accomplish what management had intended.However, there are many critiques on the manager views where unintended and negative consequences of downsizing resulted. It is important to point out that downsizing can be approached from at le ast three different perspectives: a global or industry level, a micro or individual level, and an organization or strategy level (Burnes, 2005). From a global or industrial point of view, discussions of downsizing, including mergers and acquisitions, ventures, and market strategies. From a micro or individual point of view, discussions of downsizing focus on individual stress levels associated.Hitek 7 What are some potential problems for the current class of engineers recruited at DSI? DSI does not rely on subcontractors to produce parts needed in its assembly operation, and the new employees will only receive ten hours per year of training, and most of the training will be obtained during orientation. Underutilizing these talented recruits for a long time would certainly lead to major dissatisfaction (Jackson et al; 2009, p. 226). How could the use of temporaries, student interns, or subcontractors potentially help DSI? They could bring a fresh perspective to the company.Interns of ten come in to jobs unafraid to question how things work or why jobs are performed a certain way which can help bosses discover smarter ways to do those tasks. Their perspective as newbies is valuable, especially when it comes to identifying and even solving problems that go unnoticed by full-time employees. They are at ease with the social media. Interns tend to understand social media in a way many business owners and bosses cannot, simply because young people live and breathe networks like Facebook. To them, networking on online channels is not another time-consuming task, but rather part of the day.They are productive in nature. While many full-time workers toil in jobs that lack benchmarks, most interns have a start and end date. Because of that, interns tend to be more productive. Unless you count the rare promotion or job change, some employees work in what feels like an endless cycle, which can lead to career burnout. They are courteous. New employees can be shy, but since i nterns are trying to make a good impression in a short period of time, they’re often more friendly than their paid counterparts. Interns recognize that every hallway passing could be a future opportunity.They are trying to impress. An internship Hitek 8 serves as a trial period for both sides. The intern may also be looking to set the groundwork for a recommendation or a full-time position down the road. That means they’re likely to put in a significant amount of effort to make sure you’re happy with their work. They provide free advertising via word of mouth. These young workers are excited, and if you impress them, they’ll want to tell the world about their experience with your company. They’ll spread the good word to their peers about the job, which can help recruitment efforts. They are fast learners.Young minds are like sponges, absorbing information quickly. Young workers also tend to be good at multi-tasking. The time it takes to train an in tern to perform a task is often a fraction of what it takes to train a full-time employee. Evaluate Daniels’s alternatives for reducing DSI’s labor surplus. What do you recommend? Why? Several older employees have accumulated several weeks of unused vacation time. Daniels wondered if employees could be encouraged to take unpaid leaves of absence. He also thought that early retirement incentives could be offered to make room for some of the bright young engineers (Jackson et al; 2009, p. 27). Unpaid leave is time off from work which is provided without pay. When an employee takes or is given unpaid leave, he or she retains a position in a company, and many retain benefits as well, but the employee receives no salary or wages. There are a number of reasons to take or institute this type of leave, and it is an option which is available from many companies. A company may require employees to take unpaid leave, or a furlough. This is done as a cost cutting measure, with the company preferring to force employees to take time off instead of eliminating positions at the company.This tactic is designed to retain Hitek 9 employees during periods of economic hardship, and while it may be onerous to employees, many people prefer unpaid leave to uncertain layoffs. Hitek 10 Works Cited 1. Burnes, R. (2005). Organizational Change Management: A Critical Review. Journal of Change Management Vol. 5(4): 369-380. Retrieved on March 24, 2013 from http://www. ncbi. nlm. org 2. Jackson, S. , Schuler, R. ; Werner, S. (2009). Managing Human Resources, 10th edition, pgs. 184-187, and 226-227.